TB-500 Dosage & Reconstitution Guide
Complete reference for TB-500 dosing, reconstitution math, and protocol planning. Covers vial sizes, concentrations, loading vs maintenance phases, and how it compares to BPC-157.
What TB-500 Is
TB-500 is a synthetic analogue of Thymosin Beta-4, a naturally occurring protein involved in tissue repair, cell migration, and inflammation regulation. It is widely studied in research contexts for its role in recovery processes.
Unlike short-acting peptides that are dosed multiple times daily, TB-500 has a significantly longer half-life — measured in days — making weekly or biweekly protocols the standard approach in most research frameworks.
Typical Vial Sizes and Concentrations
| Vial Size | Water Added | Concentration | 2 mg dose | 5 mg dose |
|---|---|---|---|---|
| 5 mg | 1 mL | 5 mg/mL | 0.40 mL | 1.00 mL |
| 5 mg | 2 mL | 2.5 mg/mL | 0.80 mL | 2.00 mL |
| 10 mg | 2 mL | 5 mg/mL | 0.40 mL | 1.00 mL |
| 10 mg | 4 mL | 2.5 mg/mL | 0.80 mL | 2.00 mL |
Higher water volumes produce lower concentrations and larger injection volumes per dose.
Reconstitution Workflow
- Confirm vial amount — Check the label for total mg content
- Choose a water volume — Common choices are 1–4 mL depending on target concentration
- Add bacteriostatic water — Inject slowly against the vial wall, do not spray onto powder
- Swirl gently — Do not shake; allow the powder to dissolve completely
- Calculate your draw — Use ClearDosage to convert your dose to mL or syringe units
- Label and refrigerate — Mark date, concentration, and vial ID
Dosing Math Example
Setup: 10 mg vial, 2 mL water → concentration = 5 mg/mL = 5,000 mcg/mL
Target dose: 2 mg (2,000 mcg)
- Draw = 2,000 mcg ÷ 5,000 mcg/mL = 0.40 mL
- On a U-100 syringe: 40 units
Target dose: 5 mg (5,000 mcg)
- Draw = 5,000 mcg ÷ 5,000 mcg/mL = 1.00 mL
- On a U-100 syringe: 100 units
Enter your vial size, water volume, and dose into the ClearDosage calculator to generate draw amounts for any setup.
Common Planning Approaches
Research protocols for TB-500 vary widely, but several patterns appear frequently in the literature:
Loading phase: Higher doses used for an initial period (often 4–6 weeks) followed by a maintenance phase with lower doses.
Maintenance phase: Reduced dose frequency after the loading period — commonly biweekly or monthly.
Stack context: TB-500 is frequently paired with BPC-157 in research frameworks due to their complementary tissue repair mechanisms. Both are typically dosed separately.
Individual protocols depend on research context, body weight, and goals. Consult a qualified clinician before any personal use.
TB-500 vs BPC-157: Key Differences
| TB-500 | BPC-157 | |
|---|---|---|
| Origin | Thymosin Beta-4 analogue | Gastric peptide sequence |
| Half-life | Days | Minutes to hours |
| Typical frequency | Weekly / biweekly | Once or twice daily |
| Common route | Subcutaneous | Subcutaneous or oral |
| Primary research focus | Systemic repair, inflammation | Local tissue repair, GI |
Because TB-500 works systemically and BPC-157 tends to be more site-specific in research models, they are often used together in recovery-focused protocols. ClearDosage lets you save separate configurations for each and view their half-life curves simultaneously in the Half-Life Visualizer.
Common Mistakes
Confusing mg and mcg — TB-500 doses are specified in mg (not mcg), which is larger than most peptide doses. Double-check the unit before calculating.
Using too much water — Concentrations below 1 mg/mL result in large injection volumes per dose. A 2 mL injection is generally the practical upper limit for subcutaneous delivery.
Skipping the loading phase calculation — Loading doses are typically higher than maintenance. Save separate configurations in ClearDosage for each phase to avoid drawing the wrong amount.
Not tracking vial date — TB-500 vials reconstituted in bacteriostatic water have a finite refrigerated shelf life. Use the Vial Tracker in ClearDosage to log reconstitution dates and receive expiry alerts.
Use the ClearDosage calculator to set up your TB-500 concentration once, save it, and refer back to it without recalculating from scratch each time.
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